ViroReal® Kit Bovine Coronavirus

ViroReal® Kit Bovine Coronavirus

Product beschrijving

Kit contents:

• Detection assay for bovine coronavirus
• Detection assay+target for internal RNA positive control (control of RT-PCR/RNA extraction)
• RNA reaction mix
• Nuclease-free water
• Positive control (RNA) for bovine coronavirus

Background: Bovine coronavirus (BCoV) is a member of the genus Coronavirus (Betacoronavirus), family Coronaviridae. The BCoV virion is enveloped and spherical in shape. The genome is a single-stranded, positive-sense RNA molecule. BCoV causes diarrhea in adult cattle (Winter Dysentery) and wild ruminants, as well as enteric and respiratory diseases in calves. Winter Dysentery is characterized by high morbidity (50-100%) and low mortality (1-2%), the highest incidence is between November and April when cattle are kept in close confinement. In calves morbidity and mortality are high. BCoV has been found in cattle worldwide.

Description: ViroReal^® Kit Bovine Coronavirus is based on the amplification and detection of the nucleocapsid protein gene (N gene) of the BCoV using one-step reverse transcription real-time PCR.

PCR-platforms: ViroReal^® Kit Bovine Coronavirus is developed and validated for the ABI PRISM^® 7500 instrument (Life Technologies), LightCycler^® 480 (Roche) and Mx3005P^® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Specificity and sensitivity: See product description