BactoReal® Kit Mycoplasma gallisepticum

BactoReal® Kit Mycoplasma gallisepticum

Product description

Kit contents:

• Detection assay for Mycoplasma gallisepticum
• Detection assay for internal positive control (control of amplification)
• DNA reaction mix (contains uracil-N glycosylase, UNG)
• Positive control for M. gallisepticum
• Water

Background: Avian mycoplasmosis is caused by several pathogenic mycoplasmas. Mycoplasma gallisepticum and Mycoplasma synoviae are the most important avian pathogens. In addition, Mycoplasma meleagridis and Mycoplasma iowae can also cause disease in poultry, but Mycoplasma gallisepticum and Mycoplasma synoviae are considered to be the main species of pathogenic mycoplasmas. Mycoplasma gallisepticum and Mycoplasma synoviae cause chronic respiratory disease (CRD) and infectious synovitis of chickens and turkeys especially in the presence of stresses or other respiratory pathogens, causing economic losses due to decrease in growth rate, loss of weight and reduced egg production.

Description: BactoReal^® Kit Mycoplasma gallisepticum is based on the amplification and detection of the mgc2 gene (cytadhesin-like protein encoding gene) of M. gallisepticum using real-time PCR.

PCR-platforms: BactoReal^® Kit Mycoplasma gallisepticum is developed and validated for the ABI PRISM^® 7500 instrument (Life Technologies), LightCycler^® 480 (Roche) and Mx3005P^® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Sensitivity and specificity: See product description