ProteoSpin Total Protein Concentration, Detergent Clean-Up and Endotoxin Removal Mini Kit
Product code : NOR.22800
NOR.22800For rapid and efficient endotoxin removal from proteins and peptides
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ProteoSpin Total Protein Concentration, Detergent Clean-Up and Endotoxin Removal Mini Kit
Product description
For rapid and efficient endotoxin removal from proteins and peptides
- Columns bind proteins of interest while endotoxins flow through
- Proteins are desalted
- Reduce endotoxin levels to less than 0.01 EU/µg of protein
- Greater than 95% protein recovery
- Concentrate protein samples and remove detergents at the same time
- Effectively remove a wide range of detergents including SDS, Triton® X-100, CHAPS, NP-40, and Tween 20
The ProteoSpin Total Protein Concentration, Detergent Clean-Up and Endotoxin Removal Mini Kit is also highly efficient in removing many different salts commonly used in the laboratory including, but not limited to, MgCl2, NaCl, KCl, CaCl2, LiCl and CsCl. The simultaneous removal of salts while concentrating a dilute protein solution makes the kit a convenient method for preparing proteins before running many downstream applications such as SDS-PAGE, isoelectric focusing, X-ray crystallography, NMR spectroscopy, mass spectroscopy and other applications.
The ProteoSpin Total Protein Concentration, Detergent Clean-Up and Endotoxin Removal Mini Kit is also designed for the rapid removal of endotoxins from up to 200 mg of previously purified proteins or peptides, with protein recoveries of > 95% being achieved. Endotoxins, also known as lipopolysaccharides, are cell-membrane components of Gram-negative bacteria such as E. coli. Endotoxins liberated by Gram-negative bacteria are frequent contaminations of protein solutions derived from bioprocesses. Due to the high toxicity of endotoxins in vivo and in vitro, their removal from protein preparations is often necessary prior to the use of the protein in downstream applications. This kit efficiently reduces endotoxin levels to < 0.01 EU/mg of protein, using spin column chromatography based on Norgen's proprietary resin as the separation matrix. The purified protein samples can be used in a number of downstream applications including sequencing, cloning, and in vitro and in vivo introduction into cells and organisms for various purposes.
Kit Specifications
|
|
Maximum Protein Input |
200µg
|
Maximum Column Volume Input |
600µL
|
Molecular Weight of Recovered Proteins | No molecular weight cut-off |
Final Endotoxin Levels |
< 0.01 EU/μg protein
|
Protein Recovery |
90-95%
|
% Detergent Removal | 90-95% |
Detergents that can be removed | Including Triton® X-100, CHAPS, NP-40 and Tween 20 |
Minimum Elution Volume |
50µL
|
Time to Complete 10 Purifications |
20 minutes
|
Storage Conditions
All solutions should be kept tightly sealed and stored at room temperature. All the reagents should remain stable for at least 1 year in their unopened containers.
Component | Cat. 22800 (25 preps) |
---|---|
Binding Buffer J | 8 mL |
Binding Buffer N | 4 mL |
Wash Solution M | 50 mL |
Wash Solution CIP | 20 mL |
Wash Solution N | 30 mL |
Wash Solution NIP | 20 mL |
Elution Buffer G | 6 mL |
Endotoxin Removal Solution | 1.5 mL |
Protein Neutralizer EF | 2 mL |
Mini Spin Columns | 25 |
Collection Tubes | 25 |
Elution Tubes (1.7 mL) | 25 |
Product Insert | 1 |
ProteoSpin Total Protein Concentration, Detergent Clean-Up and Endotoxin Removal Mini Kit
Product specifications
Packaging Unit
25 preps
Brand
Norgen biotek
Category
Protein Purification Kits
Purification
Protein Clean-Up, Concentration and Buffer Exchange
ProteoSpin Total Protein Concentration, Detergent Clean-Up and Endotoxin Removal Mini Kit
Product documents