Stool DNA Isolation Kit
Code produit : NOR.27600
NOR.27600For the rapid and simple purification of bacterial and host DNA from stool and fecal samples

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Stool DNA Isolation Kit
Product Description
For the rapid and simple purification of bacterial and host DNA from stool and fecal samples
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Simultaneous isolation of both host DNA and microbial DNA (universal protocol)
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Eliminates PCR inhibitors including humic acids
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Fully compatible with Norgen's Stool Nucleic Acid Collection and Transport Tubes
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High quality DNA for sensitive downstream applications including PCR, qPCR, Sequencing and microarray
This kit provides a convenient and rapid method to isolate total DNA from fresh, frozen and preserved stool samples, including those preserved using Norgen's Stool Nucleic Acid Collection and Preservation Tubes (Cat. 45660). The universal protocol conveniently allows for the isolation of total genomic DNA from all the various microorganisms and host cells found in the stool sample simultaneously. The kit removes all traces of humic acids and other inhibitors using Bead Tubes and a combination of chemical and physical homogenization and lysis, without the need of any phenol-chloroform extractions. A simple and rapid spin column procedure is then used to further purify the DNA with high yields and molecular weights of up to 50 kb plus. The purified DNA is of the highest quality and is fully compatible with all downstream applications such as PCR, qPCR, Sequencing and microarrays since all humic acid substances and other PCR inhibitors are removed during the isolation process.
Kit Specifications
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Maximum Stool Input
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200 mg
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Type of Stool Processed
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Frozen, fresh or preserved stool
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Format | Spin Column |
Maximum Column Binding Capacity
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50µg
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Maximum Column Loading Volume |
650µL
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Elution Volume | 50µL |
Time to Complete 10 Purifications |
30 minutes
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Applications | PCR, qPCR, Southern Blot Analysis, |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.
Component | Cat. 27600 (50 preps) |
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Lysis Buffer L | 60 mL |
Lysis Additive A | 6 mL |
Binding Buffer I | 7 mL |
Binding Buffer C | 30 mL |
Wash Solution A | 18 mL |
Elution Buffer B | 8 mL |
Bead Tube | 50 |
Mini Spin Columns | 50 |
Collection Tubes | 50 |
Elution Tubes (1.7 mL) | 50 |
Product Insert | 1 |
Stool DNA Isolation Kit
Spécifications du produit
Packaging Unit
50 preps
Brand
Norgen biotek
Category
Stool Purification
Purification
DNA Purification
Stool DNA Isolation Kit
Documents de produit