Cytoplasmic & Nuclear RNA Purification Kit
Product code : NOR.21000 NOR.21000
For the convenient purification of cytoplasmic and nuclear RNA from cultured cells and tissues
Packaging Unit
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Cytoplasmic & Nuclear RNA Purification Kit
Product description
For the convenient purification of cytoplasmic and nuclear RNA from cultured cells and tissues
- Excellent separation and purification of cytoplasmic and nuclear RNA
- Convenient and fast spin column format
- High quality and yield of RNA
- Isolate full diversity of RNA (including microRNA) without phenol
- Purified RNA is ready for any application including RT-PCR, qRT-PCR, RNA-Seq, arrays and more
- Cytoplasmic RNA is free of DNA and ready for direct use in RT-PCR/qRT-PCR
This kit provides a rapid method for the isolation and purification of both cytoplasmic and nuclear RNA from cultured animal cells and small tissue samples. The kit can be used to isolate all sizes of RNA from the cytoplasmic and nuclear RNA fractions, including all small RNA species without any requirement for phenol. Included in the kit are sufficient reagents to perform either 50 cytoplasmic RNA preparations or 25 cytoplasmic and 25 nuclear RNA preparations. Ten samples can be processed in approximately 45 minutes. This kit is also available in a 100 prep size.
Background
In certain circumstances it is desirable to be able to isolate fractionated RNA as opposed to total RNA. For example, it may be preferable to isolate only mature, cytoplasmic RNA for some studies on expression profiling. Alternatively it may be desirable to isolate nuclear RNA in order to investigate and study pre-processed (non-spliced) RNA. Furthermore, this kit can be used to isolate RNA for downstream applications where it is necessary to avoid DNA contamination, since the cytoplasmic fraction has been shown to be free of all traces of genomic DNA.
Kit Specifications
|
|
Binding Capacity Per Column
|
Up to 50 µg RNA
|
Maximum Loading Volume Per Spin Column
|
650 µL
|
Size of RNA Purified
|
All sizes, including < 200 nt
|
Time to Complete 10 Purifications |
45 minutes
|
RNA Yield
HeLa (1 x 10 6) - Cytoplasmic RNA HeLa (1 x 10 6) - Nuclear RNA |
15 µg
< 3.5 µg |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.
Component | Cat. 21000 (50 preps) | Cat. 37400 (100 preps) |
---|---|---|
Lysis Buffer J | 20 mL | 2 x 20 mL |
Buffer SK | 40 mL | 2 x 40 mL |
Wash Solution A | 38 mL | 2 x 38 mL |
Elution Buffer E | 6 mL | 2 x 6 mL |
Mini Spin Columns | 50 | 100 |
Collection Tubes | 50 | 100 |
Elution Tubes (1.7 mL) | 50 | 100 |
Product Insert | 1 | 1 |
Cytoplasmic & Nuclear RNA Purification Kit
Product specifications
Packaging Unit
50 preps
Brand
Norgen biotek
Category
Cytoplasmic and Nuclear RNA and microRNA Purification
Purification
RNA Purification