PCR Purification Kit

Product code : NOR.14400 NOR.14400

For the rapid purification of amplified DNA products from PCR mixes

Packaging Unit

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PCR Purification Kit

Product description

For the rapid purification of amplified DNA products from PCR mixes

Purify amplified DNA ranging from 100 bp -15,000 bp in size
Fast and efficient spin column format
Available in a 50 prep size and a 250 prep size
Also available in 96 well format

This kit enables the rapid purification of amplified DNA products from PCR mixes. It is able to effectively remove PCR by-products including primers, dimers, enzymes, unincorporated nucleotides and mineral oil from the desired PCR product. The purified PCR products are fully compatible with restriction enzyme digestion, ligation into vectors, labeling, sequencing and more. This kit can also be used as an alternative to organic extraction and ethanol precipitation to clean up various enzymatic reactions.

The kit is also available in a 96-well format for high-throughput PCR purification. Purification with the 96-well plate can be performed using either a vacuum manifold or centrifugation.

Kit Specifications - Spin Column
Column Binding Capacity	10µg
Size of DNA Purified	100 - 15,000 bp
Average Recovery	> 90%
Average Primer Removal	> 90%
Minimum Elution Volume	30µL
Time to Complete 10 Purifications	15 minutes

Kit Specifications - 96-well
Binding Capacity Per Well	15µg
Maximum Loading Volume Per Well	500µL
Size of DNA Purified	100 - 15,000 bp
Average DNA Recovery	> 90%
Average Primer Reoval	> 90%
Minimum Elution Volume	50µL
Time to Complete 96 Purifications	20 minutes

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.

Component	Cat. 14400 (50 preps)	Cat. 45700 (250 preps)
Binding Buffer C	30 mL	5 x 30 mL
Wash Solution A	12 mL	2 x 20 mL
Elution Buffer B	8 mL	2 x 30 mL
Spin Columns	50	250
Collection Tubes	50	250
Elution Tubes (1.7 mL)	50	250
Product Insert	1	1

Component	Cat. 24800 (2 x 96-well)
Binding Buffer C	3 x 30 mL
Wash Solution A	2 x 38 mL
Elution Buffer B	2 x 15 mL
96-Well Plate	2
Adhesive Tape	4
96-Well Collection Plate	2
96-Well Elution Plate	2
Product Insert	1

PCR Purification Kit

Product specifications

Packaging Unit

50 preps

Brand

Norgen biotek