Plasmid MiniPrep Kit

Product code : NOR.13300 NOR.13300

For rapid and convenient plasmid DNA preparations

Packaging Unit

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Plasmid MiniPrep Kit

Product description

For rapid and convenient plasmid DNA preparations

High yield of plasmid DNA
Purify plasmids up to 13 kb
Plasmid DNA is ready for restriction digestion, bacterial transformation, sequencing and more
Rapid spin-column format and 96-well format available

This kit is designed for the rapid preparation of plasmid DNA from small cultures of Escherichia coli using convenient spin columns. The plasmid DNA is preferentially purified from other cellular components such as genomic DNA and RNA. This kit is able to purify plasmids up to 13,000 bp in size, and the typical purification yield is up to 20µg from 1.5 mL of bacterial culture. Purified DNA is of excellent quality for transformation, restriction enzyme digestion, sequencing and more. Also available in a 96-well format.

Kit Specifications
Column Binding Capacity	25 µg
Size of Plasmids Purified	Up to 13,000 bp
Average Yield from 1.5 mL of Culture	Up to 20 µg
Time to Complete 10 Purifications	30 minutes

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers. The RNAse should be stored at -20°C upon arrival. The Resuspension Buffer should be stored at 4°C upon addition of RNAse enzyme.

Component	Cat. 13300 (50 preps)	Cat. 46400 (250 preps)
Resuspension Solution AZ	12 mL	60 mL
Lysis Buffer N	40 mL	80 mL
Buffer TN	20 mL	130 mL
Wash Solution E	12 mL	2 x 18 mL
Elution Buffer K	8 mL	30 mL
RNase A	1 vial	1 vial
Spin Columns	50	250
Collection Tubes	50	250
Elution Tubes (1.7 mL)	50	250
Product Insert	1	1

Component	Cat. 25900 (96-well)
Resuspension Buffer	24 mL
Lysis Solution	30 mL
Binding Solution	40 mL
Wash Solution	12 mL
Elution Buffer	20 mL
RNAse	1 vial
96-Well Deep Plate	1
96-Well Filter Plate	1
96-Well Plate	1
Adhesive Tape	6
96-Well Collection Plate	2
96-Well Elution Plate	1
Product Insert	1

Plasmid MiniPrep Kit

Product specifications

Packaging Unit

50 preps

Brand

Norgen biotek