SampleIN™ Direct PCR Kit

SampleIN™ Direct PCR Kit

Product description

SampleIN™ Direct PCR Kit is a premium tool for a fast direct PCR eliminating the need of tedious template purification. The kit is excellent for direct PCR from mouse tail or ear, mammalian tissues, hair follicle, buccal swabs and blood.

Rapid 15 min DNA extraction using DPK Lysis and Protease Buffers in a single tube generates PCR template extract which is further amplified under fast cycling conditions with a hot-start Taq master mix that includes red dye for direct gel loading.

In a 2% agarose TAE gel the red dye migrates with~350 bp DNA, in 1% agarose TAE gel with ~ 600 bp DNA fragments.

The ALLin™ HS Red Taq Mastermix includes a hot start Taq DNA Polymerase what ensures high yield, specific, low background amplification. Mix components allow for a fast PCR cycling and increase success when working with complex templates or multiplexing. Generated A-tailed PCR products are suitable for ligating into TA cloning vectors, sequencing and other applications.

Applications

• Fast direct PCR without template purification
• Mouse genotyping and knockout analysis
• Direct PCR from mouse tail or ear, mammalian tissues (including FFPE)
• Direct PCR from hair follicle, buccal swabs, plant samples
• Direct Blood PCR (including EDTA or FTA samples)

Benefits

• Ready-to load PCR in 50 minutes without template purification
• Single-tube 15 min DNA extraction combined with fast hot-start PCR
• Red dye in the PCR master mix for direct gel loading
• High yields under standard or fast cycling conditions
• Success with GC/AT rich templates