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Total RNA Purification Kit
Product code : NOR.17200 NOR.17200
For rapid purification of total RNA - including microRNA - without phenol
Packaging Unit
You can contact us for more information via the button “quote request'
Total RNA Purification Kit
Product description
For rapid purification of total RNA - including microRNA - without phenol
- Extract high quality & purity total RNA including miRNA
- No phenol step required
- Bind & elute all RNA irrespective of size or GC content, without bias
- Efficiently extract small RNA irrespective of GC content
- Very sensitive & linear down to a few cells without the need for carrier RNA
- Convenient & fast spin column format
- Isolate from a wide variety of specimens
This kit is suitable for the isolation of total RNA from a range of samples including cells, bacteria, yeast, virus and bodily fluids including plasma/serum, blood, saliva, CSF and more. Extract high quality and purity RNA with excellent RIN values and A260/A280 suitable for downstream applications including qRT-PCR, RT-PCR, microarrays, NGS and more.
The kit purifies all sizes of RNA from large mRNA, lncRNA down to microRNA (miRNA) in the same fraction without the requirement of phenol. Isolate all RNA sequences at an equal rate irrespective of size. Moreover, when the RNA sequences are small (e.g. miRNA), the column binds small RNAs regardless of their GC content.
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Kit Specifications
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Binding Capacity Per Column
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Up to 50µg RNA
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Maximum Loading Volume Per Spin Column
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650µL
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Size of RNA Purified
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All sizes, including < 200 nt
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| Time to Complete 10 Purifications |
20 minutes
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Maximum Amount of Starting Material
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Liver (10 mg)
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30 µg
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Kidney (10 mg)
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10 µg
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Brain (10 mg)
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12 µg
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| Blood (hamster, 100µL |
5 µg
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HeLa (1 x 10
6)
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15 µg
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CHO (1 x 10
6)
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11 µg
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Yeast (1 x 10
8)
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30 µg
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| E. coli (1 x 109) |
43 µg
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Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.
| Component | Cat. 17200 (50 preps) | Cat. 37500 (100 preps) |
|---|---|---|
| Buffer RL | 40 mL | 2 x 40 mL |
| Wash Solution A | 38 mL | 2 x 38 mL |
| Elution Solution A | 6 mL | 2 x 6 mL |
| Mini Spin Columns | 50 | 100 |
| Collection Tubes | 50 | 100 |
| Elution Tubes (1.7 mL) | 50 | 100 |
| Product Insert | 1 | 1 |
Total RNA Purification Kit
Product specifications
Packaging Unit
50 Preps
Brand
Norgen biotek
Category
Total RNA Extraction and Purification (including microRNA)
Purification
RNA Purification