ViroReal® Kit Bovine Norovirus

ViroReal® Kit Bovine Norovirus

Product description

Kit contents:

• Detection assay for bovine norovirus
• Detection assay+target for internal RNA positive control (control of RT-PCR/RNA extraction)
• RNA reaction mix
• Nuclease-free water
• Positive control (RNA) for bovine norovirus

Background: Noroviruses are non-enveloped, single-stranded, positive-sense RNA viruses ((+)ssRNA), belonging to the family Caliciviridae. Strains are classified in 5 genotypes (GIGV), infecting different species, including human, bovine, swine, mice and oysters. Bovine norovirus belong to GIII. Norovirus GIII includes two genotypes (GIII.1 and GIII.2), with genotype GIII.1 represented by Jena virus and GIII.2 represented by Newbury-2 virus. Infection is associated with gastrointestinal manifestations such as diarrhea, villus atrophy and crypt hyperplasia of the proximal small intestine in cattle, especially in young calves.

Description: ViroReal^® Kit Bovine Norovirus is based on the amplification and detection of the ORF1 of so far known bovine norovirus genotypes GIII.1 and GIII.2 using one-step reverse transcription real-time PCR.

PCR-platforms: ViroReal^® Kit Bovine Norovirus is developed and validated for the ABI PRISM^® 7500 instrument (Life Technologies), LightCycler^® 480 (Roche) and Mx3005P^® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Specificity and sensitivity: See product description