Rugini Olive Medium

Rugini Olive Medium

Product Description

The Olive (Olea europaea sativa L.) plays an important role in the economies of countries in the Mediterranean area. The in vitro culture of Olive, a particular difficult species to propagate in vitro, required the development of a specific medium formulation. Rugini medium is dedicated to the proliferation of the olive shoots. The medium has an enriched composition compared to MS. Olive tissues are characterized by a high content of Ca, Mg, S, Cu and Zn. The best nitrogen source is a combination of NO3- and NH4+ supplemented with glutamine 2,19 mg/l. The better carbon source is mannitol (30-36 gr/l) compared to sucrose. A better cytokinin to be used is zeatin: 1 mg/l if filter sterilized, 3-4 mg/l when autoclaved. TDZ and 2iP are less effective. Shoots grow more rapidly compared to other media. The proliferation rate increases and more tender, sturdier shoots with less basal callus are obtained.

Composition

Micro Elements	mg/l	µM
CoCl2.6H2O	0.025	0.11
CuSO4.5H2O	0.25	1.00
FeNaEDTA	36.70	100.00
H3BO3	12.40	200.55
KI	0.83	5.00
MnSO4.H2O	16.90	100.00
Na2MoO4.2H2O	0.25	1.03
ZnSO4.7H2O	14.30	49.75

Macro Elements	mg/l	mM
CaCl2	332.16	2.99
Ca(NO3)2	416.92	2.54
KCl	500.00	6.71
KH2PO4	340.00	2.50
KNO3	1100.00	10.88
MgSO4	732.60	6.09
NH4NO3	412.00	5.15

Vitamins	mg/l	µM
Biotin	0.05	0.20
Folic acid	0.50	1.13
Glycine	2.00	26.64
myo-Inositol	100.00	554.94
Nicotinic acid	5.00	40.62
Pyridoxine HCl	0.50	2.43
Thiamine HCl	0.50	1.48

Total concentration Micro and Macro elements including vitamins: 4023.89 mg/l

Rugini E., In vitro propagation of some olive cultivars, Scientia Horticulturae 24, 123 (1984)., Jacoboni A., Luppino M., Rugini E., Role of basal shoot darkening Scientia Horticolturae, 53:63 (1993)