Gamborg B5 medium including vitamins

Gamborg B5 medium including vitamins

Product beschrijving

B5 medium has been defined for the growth of cell suspensions of soybean root cells in the presence of 2,4 D. Nitrate was required in a concentration of 20-30 mM. An addition of 2 mM ammoniumsulphate led to an increase in cellgrowth. NH4+ when added as the sole source of nitrogen, did not support growth. Similar results were obtai-ned when NH4NO3 was substi-tuted for (NH4)2SO4. However, ammonium ions depressed growth when the concen-tration excee-ded 2 mM. Variati-ons in the concentrations of phosphate, calcium and magnesi-um resulted in relatively minor chan-ges in growth rate. Thiamine is known to be an essenti-al nutrient for cell growth and is increa-sed in concentrati-on up to 10 mg/l. B5 medium has been defined for the growth of cell suspensions of soybean root cells in the presence of 2,4 D. Nitrate was required in a concentration of 20-30 mM. An addition of 2 mM ammoniumsulphate led to an increase in cellgrowth. NH4+ when added as the sole source of nitrogen, did not support growth. Similar results were obtai-ned when NH4NO3 was substi-tuted for (NH4)2SO4. However, ammonium ions depressed growth when the concen-tration excee-ded 2 mM. Variati-ons in the concentrations of phosphate, calcium and magnesi-um resulted in relatively minor chan-ges in growth rate. Thiamine is known to be an essenti-al nutrient for cell growth and is increa-sed in concentrati-on up to 10 mg/l.

Composition

Micro Elements	mg/l	µM
CoCl2.6H2O	0.025	0.11
CuSO4.5H2O	0.025	0.10
FeNaEDTA	36.70	100.00
H3BO3	3.00	48.52
KI	0.75	4.52
MnSO4.H2O	10.00	59.16
Na2MoO4.2H2O	0.25	1.03
ZnSO4.7H2O	2.00	6.96

Macro Elements	mg/l	mM
CaCl2	113.23	1.02
KNO3	2500.00	24.73
MgSO4	121.56	1.01
NaH2PO4	130.44	1.09
(NH4)2SO4	134.00	1.01

Vitamins	mg/l	µM
myo-Inositol	100.00	554.94
Nicotinic acid	1.00	8.12
Pyridoxine HCl	1.00	4.86
Thiamine HCl	10.00	29.65

Total concentration Micro and Macro elements including -vitamins: 3163.98 mg/l

Gamborg O.L., Miller R.A., Ojima K., Nutrient requirement of suspensions cultures of soybean root cells. Exp. Cell Res., 50, 151 (1968).