Murashige & Skoog medium including vitamins

Murashige & Skoog medium including vitamins

Product beschrijving

MS medium is the most used tissue culture medium, of which many variations have been developed. The medium is originated from White's medium and originally developed for the cultivation of Nicotiana tabacum calli. Compared to White medium, the concentration of all ingredients is increased. An increase to 50-60 mM nitrogen stimulated the growth of Nicotiana cells significantly, however a concentration of 80 mM and higher was clearly disadvantageous to the cells. The increase of all other elements, especially the macro -elements, also stimulated the growth of the calli. Due to the high concentration of minerals, MS medium is a very rich and -saline -medium and may be too salty to certain plant species. To avoid this problem, MS is often used with the micro elements in full concentration, but with the macro elements in respectively half or three-quarter of the concentration as originally described by the authors. Sometimes the original MS vitamines are replaced by the vitamins of Linsmaier and Gamborg B5 medium regarding the higher concentration of Thiamine in relation to the requirement of this vitamin by plants.

Composition

Micro Elements	mg/l	µM
CoCl2.6H2O	0.025	0.11
CuSO4.5H2O	0.025	0.10
FeNaEDTA	36.70	100.00
H3BO3	6.20	100.27
KI	0.83	5.00
MnSO4.H2O	16.90	100.00
Na2MoO4.2H2O	0.25	1.03
ZnSO4.7H2O	8.60	29.91

Macro Elements	mg/l	mM
CaCl2	332.02	2.99
KH2PO4	170.00	1.25
KNO3	1900.00	18.79
MgSO4	180.54	1.50
NH4NO3	1650.00	20.61

Vitamins	mg/l	µM
Glycine	2.00	26.64
myo-Inositol	100.00	554.94
Nicotinic acid	0.50	4.06
Pyridoxine HCl	0.50	2.43
Thiamine HCl	0.10	0.30

Total concentration Micro and Macro elements including vitamins: 4405.19 mg/l

Murashige T. and Skoog F., Physiol. Plant, 15, 473 (1962).