Plant/Fungi Total RNA Purification Kit

Product code : NOR.31350 NOR.31350

For the rapid purification of total RNA (including microRNA) from plants and fungi

Packaging Unit

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Plant/Fungi Total RNA Purification Kit

Product beschrijving

For the rapid purification of total RNA (including microRNA) from plants and fungi

Extract total RNA, including virus & viroid RNA
Robust lysis buffer is well-suited to even challenging samples such as pine needle, grape leaf, etc
Isolate total RNA (including microRNA) without phenol
Isolated RNA is of high quality, integrity and diversity
Also available in 96-well format for high throughput applications

Norgen's Plant/Fungi Total RNA Purification Kit provides a rapid method for the isolation and purification of total RNA, including virus and viroid RNA, from a wide range of plants. Total RNA can be purified from fresh or frozen plant tissues, plant cells or filamentous fungi samples using this kit. All sizes of RNA are purified, including microRNA (miRNA) . The procedure is rapid and convenient.

The RNA is purified without the use of phenol or chloroform. The purified RNA is of the highest quality, and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.

Norgen's Plant/Fungi Total RNA Purification Kit is also available in a 96-well format for high throughput applications. Purification with the 96-well plates can be performed using either a vacuum manifold or centrifugation.

Kit Specifications - Spin Column
Column Binding Capacity	50µg
Maximum Column Loading Volume	650µL
Size of RNA Purified	All sizes, including small RNA (< 200 nt)
Maximum Amount of Starting Material: Plant Tissues Plant Cells Fungi	50 mg 1 x 10 ⁶ cells 50 mg (wet weight)
Average Yields* 50 mg Tomato Leaves 50 mg Tobacco Leaves 50 mg Plum Leaves 50 mg Grape Leaves 50 mg Peach Leaves	60µg 60µg 32µg 35µg 30µg
Time to Complete 10 Purifications	30 minutes

* Yield will vary depending on the type of sample processed.

Kit Specifications - 96-well
Binding Capacity Per Well	50µg
Maximum Loading Volume Per Well	500µL
Size of RNA Purified	All sizes, including small RNA (< 200 nt)
Maximum Amount of Starting Material: Fungi Plant Tissues	40 mg 40 mg
Average Yields* 25 mg of Grape Leaves 40 mg Tomato Leaves 40 mg Tobacco Leaves 40 mg Peach Leaves	5-7µg 20-30µg 20-30µg 15-20µg
Time to Complete 96 Purifications	30 minutes

* Yield will vary depending on the type of sample processed.

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.

Select Plants and Fungi Tested with the Norgen Plant/Fungi Total RNA Purification Kits

Plants

Tobacco (Nicotiana tabacum)
Tomato (Lycopersicon esculentum)
Pepper (Capsicum annuum)
Potato (Solanum tuberosum)
Arabidopsis thaliana1
Peach (Prunus persica)
Apple (Malus sp.)
Pear (Pyrus sp.)
Grape vine (Vitis sp.)
Plum (Prunus sp.)
Palm (Arecaceae)
Pine needle (Pinaceae)
Strawberry
Raspberry
Blackberry
Herbs
Persimmon (Ebenaceae)
Potato tuber (Solanum)
Plum fruit
Citrus
Vanilla bean
Cotton (Gossypium)
Mangrove
Chrysanthemum
Grape berry skin
Kiwi leaves
Peach (fruits and flowers)
Soy bean (legume)
Eastern White Red Cedar
Corn leaves
Cucumber leaves

Fungi

Aspergillus niger
Mucor racemosus
Cladosporium cladosporioides
Fusarium oxysporum
Penicillium sp.
Botrytis cinerea (Botryotinia fuckeliana)
Pichia sp.
Rhizopus oryzae
Alternaria tenuissima

Component	Cat. 25800 (50 preps)	Cat. 31350 (100 preps)
Lysis Buffer C	60 mL	1 x 20 mL, 1 x 60 mL
Wash Solution A	38 mL	38 mL
Elution Solution A	6 mL	6 mL
Filter Columns	50	100
Spin Columns	50	100
Collection Tubes	100	200
Elution Tubes (1.7 mL)	50	100
Product Insert	1	1

Component	Cat. 31900 (2 x 96-well)
Lysis Solution	2 x 40 mL
Wash Solution	2 x 40 mL
Elution Solution	20 mL
96-Well Filter Plate	2
Adhesive Tape	4
96-Well Collection Plate	2
96-Well Elution Plate	2
Product Insert	1

Plant/Fungi Total RNA Purification Kit

Product specificaties

Packaging Unit

100 preps

Brand

Norgen biotek