|
|
|
Geen items |
Plasmid MiniPrep Kit
Product code : NOR.25900 NOR.25900
For rapid and convenient plasmid DNA preparations
Packaging Unit
Via de knop “offerte aanvraag” kan u vrijblijvend contact met ons opnemen voor verdere informatie.
Plasmid MiniPrep Kit
Product beschrijving
For rapid and convenient plasmid DNA preparations
- High yield of plasmid DNA
- Purify plasmids up to 13 kb
- Plasmid DNA is ready for restriction digestion, bacterial transformation, sequencing and more
- Rapid spin-column format and 96-well format available
This kit is designed for the rapid preparation of plasmid DNA from small cultures of Escherichia coli using convenient spin columns. The plasmid DNA is preferentially purified from other cellular components such as genomic DNA and RNA. This kit is able to purify plasmids up to 13,000 bp in size, and the typical purification yield is up to 20µg from 1.5 mL of bacterial culture. Purified DNA is of excellent quality for transformation, restriction enzyme digestion, sequencing and more. Also available in a 96-well format.
|
Kit Specifications
|
|
| Column Binding Capacity |
25 µg
|
| Size of Plasmids Purified |
Up to 13,000 bp
|
| Average Yield from 1.5 mL of Culture |
Up to 20 µg
|
| Time to Complete 10 Purifications |
30 minutes
|
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers. The RNAse should be stored at -20°C upon arrival. The Resuspension Buffer should be stored at 4°C upon addition of RNAse enzyme.
| Component | Cat. 13300 (50 preps) | Cat. 46400 (250 preps) |
|---|---|---|
| Resuspension Solution AZ | 12 mL | 60 mL |
| Lysis Buffer N | 40 mL | 80 mL |
| Buffer TN | 20 mL | 130 mL |
| Wash Solution E | 12 mL | 2 x 18 mL |
| Elution Buffer K | 8 mL | 30 mL |
| RNase A | 1 vial | 1 vial |
| Spin Columns | 50 | 250 |
| Collection Tubes | 50 | 250 |
| Elution Tubes (1.7 mL) | 50 | 250 |
| Product Insert | 1 | 1 |
| Component | Cat. 25900 (96-well) | |
| Resuspension Buffer | 24 mL | |
| Lysis Solution | 30 mL | |
| Binding Solution | 40 mL | |
| Wash Solution | 12 mL | |
| Elution Buffer | 20 mL | |
| RNAse | 1 vial | |
| 96-Well Deep Plate | 1 | |
| 96-Well Filter Plate | 1 | |
| 96-Well Plate | 1 | |
| Adhesive Tape | 6 | |
| 96-Well Collection Plate | 2 | |
| 96-Well Elution Plate | 1 | |
| Product Insert | 1 |
Plasmid MiniPrep Kit
Product specificaties
Packaging Unit
1 x 96-well plate
Brand
Norgen biotek
Category
Plasmid Purification Kits
Purification
DNA Purification