ALLin™ Red Taq Mastermix

ALLin™ Red Taq Mastermix

Product beschrijving

highQu ALLin™ Taq DNA Polymerase is the versatile engineered enzyme which in combination with the optimized ALLin™ buffer provides higher success rates in  demanding PCR applications like amplification of complex templates, crude sample PCR and fast cycling. ALLin™ Taq DNA Polymerase has the same PCR accuracy like Taq DNA Polymerase, 4.5 x 10⁴ (a number of correct nucleotides incorporated before the error occurs) and produces A-tailed products suitable for ligating into TA cloning vectors.

The convenience of ALLin™ Taq DNA Polymerase is maximized by the use of 2X Red Mastermix providing the additional advantage of reduced pipetting and minimized errors.

ALLin™ Red Taq Mastermix, 2X is premixed with red dye and density reagents for direct loading on the gels after the PCR.

In a 2% agarose TAE gel the dye migrates with~350 bp DNA, in 1% agarose TAE gel with ~ 600 bp DNA fragments.

The mastermix is even supplied with the PCR Water and the only thing to add is the template with primers.

Applications

• Routine PCR up to 6 kb with a direct gel loading option
• PCR of GC rich templates
• Colony PCR, Fast PCR, TA cloning

Benefits

• Premixed with the dye and density reagent for direct loading on the gels
• Higher yields under standard and fast cycling
• Success with longer (6 kb) and GC rich templates