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Leukocyte RNA Purification Kit
Product code : NOR.21200
NOR.21200For the rapid extraction and purification of total RNA from leukocytes
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Leukocyte RNA Purification Kit
Product description
For the rapid extraction and purification of total RNA from leukocytes
- Fractionate leukocytes from whole blood in minutes with provided RBC Lysis Buffer
- Isolate total RNA, including microRNA, without phenol
- Rapid and convenient spin-column format
- Purified RNA is ready for any downstream application including RT-PCR, qRT-PCR, NGS, arrays and more
This kit provides a rapid method for the isolation and purification of total leukocyte RNA from mammalian blood samples in 40 minutes. The kit is supplied with an RBC (red blood cell) Lysis Buffer for selective removal of red blood cells and fractionation of leukocytes by centrifugation. Isolation of leukocyte RNA results in improved expression profiling and other downstream applications by removing the masking effects of some RNAs which are very abundant in whole blood, such as globin mRNAs. The kit is able to isolate total leukocyte RNA, including both large mRNA and all small RNA species containing microRNA (miRNA) and small silencing RNA (siRNA). The purified RNA is of the highest quality and can be used in a number of downstream applications.
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Kit Specifications
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| Binding Capacity Per Spin Column |
Up to 50µg RNA
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| Maximum Loading Volume Per Spin Column |
650µL
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| Size of RNA Purified |
All sizes, including <200 nt
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| Minimum Blood Input Volume |
10µL
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| Maximum Blood Input Volume |
2 mL of 3 x 10
6 leukocytes
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| Time to Complete 10 Purifications |
40 minutes
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| Input Type |
Blood from Human, Hamster, Mouse, Rabbit, and Rat
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| Average Yields Human Blood (500µL) Hamster Blood (100µL) |
1.5µg
2.5µg |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. The RBC Lysis Buffer should be stored at 4°C upon arrival. These reagents should remain stable for at least 1 year in their unopened containers.
| Component | Cat. 21200 (50 preps) |
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| RBC Lysis Buffer | 2 x 100 mL |
| Buffer RL | 30 mL |
| Wash Solution A | 38 mL |
| Elution Solution A | 6 mL |
| Mini Spin Columns | 50 |
| Collection Tubes | 50 |
| Elution Tubes (1.7 mL) | 50 |
| Product Insert | 1 |
Leukocyte RNA Purification Kit
Product specifications
Packaging Unit
50 preps
Brand
Norgen biotek
Category
Blood/Plasma/Serum Sample Preparation Kits
Purification
RNA Purification