Urine DNA Isolation Kit
Product code : NOR.18100
NOR.18100Fast and reliable purification of genomic and apoptotic DNA from urine
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Urine DNA Isolation Kit
Product description
Fast and reliable purification of genomic and apoptotic DNA from urine
- Rapid isolation of both small and large species of DNA from urine
- Convenient spin column format
- Effective removal of PCR inhibitors
- Purified DNA is highly suited to sensitive downstream applications
- Allows for the purification of viral DNA from urine
- Small urine input ranging from as low as 50 µL to 1.75 mL
This kit provides a fast, reliable and simple procedure for isolating DNA from up to 1.75 mL of urine. Both high molecular weight DNA (greater than 1 kb in size; mostly cell associated) and the smaller DNA (150 - 250 bp; derived from the circulation) is effectively isolated and purified using a rapid and convenient spin column protocol. Multiple samples can be processed in 30 minutes. This kit can be used to isolate DNA from a broad range of viruses in urine as well. Salts, metabolic wastes, proteins and other contaminants are removed to yield inhibitor-free DNA for use in sensitive applications. The DNA is of excellent quality for various downstream applications such as PCR, qPCR and DNA fingerprinting, methylation studies and more.
This kit is fully compatible with Norgen's Urine Collection and Preservation Tubes.
Background
DNA found in urine can be divided into 2 basic categories. The larger species, genomic-DNA (gDNA), is generally greater than 1 kb in size, and appears to be derived mainly from exfoliated cells. The second species is smaller, generally between 150 and 250 bp (apoptotic-DNA), and derives, at least in part, from the circulation. The second species is also considered as an RNA/DNA hybrid as reported by Halicka et al. (2000). Both types of DNA can be isolated reliably using this kit.
Kit Specifications
|
|
Volume of Urine Processed
|
1.75 mL
|
Average Yield*
|
Up to 50 ng
|
Size of DNA Purified
|
Large (> 1 kb)
and small (150-250 bp) |
Time to Complete 10 Purifications |
30 minutes hands-on time
(plus a 1 hour incubation) |
* Yield will vary depending on the type of sample processed
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.
Norgen's Urine DNA Isolation Kit contains ready-to-use Proteinase K and Pronase solutions, which are dissolved in a specially prepared storage buffer. The Proteinase K and the Pronase are stable for up to 1 year after delivery when stored at room temperature. To prolong the lifetime of Proteinase K and Pronase, storage at 2-8°C is recommended.
Component | Cat. 18100 (50 preps) |
---|---|
Binding Solution K | 15 mL |
Proteinase K in Storage Buffer | 2 mL |
Pronase K in Storage Buffer | 2 mL |
Soluton WN | 9 mL |
Wash Solution D | 30 mL |
Wash Solution B | 15 mL |
Micro Spin Columns | 50 |
Collection Tubes | 50 |
Elution Tubes (1.7 mL) | 100 |
Product Insert | 1 |
Urine DNA Isolation Kit
Product specifications
Packaging Unit
50 preps
Brand
Norgen biotek
Category
Urine DNA Purification Kits
Purification
DNA Purification