NLN medium

NLN medium

Product description

The composition of the NLN medium originated from the medium described by Nitsch. The medium was developed for anthercultures of Brassica Napus in liquid medium and the induction of haploid plants from isolated pollen. NLN medium is provided free of Calcium nitrate. In the original medium Ca(NO3)2.4H2O is present in a concentration of 500 mg/l. To prepare the proper NLN medium formulation 500 mg/l Ca(NO3)2.4H2O has to be added extra to the already dissolved powdered medium.The composition of the NLN medium originated from the medium described by Nitsch. The medium was developed for anthercultures of Brassica Napus in liquid medium and the induction of haploid plants from isolated pollen. NLN medium is provided free of Calcium nitrate. In the original medium Ca(NO3)2.4H2O is present in a concentration of 500 mg/l. To prepare the proper NLN medium formulation 500 mg/l Ca(NO3)2.4H2O has to be added extra to the already dissolved powdered medium.

Composition

Micro Elements	mg/l	µM
CoCl2.6H2O	0.025	0.11
CuSO4.5H2O	0.025	0.10
FeNaEDTA	36.70	100.00
H3BO3	10.00	161.73
MnSO4.H2O	18.95	111.94
Na2MoO4.2H2O	0.25	1.03
ZnSO4.7H2O	10.00	34.78
CoCl2.6H2O	0.025	0.11
CuSO4.5H2O	0.025	0.10
FeNaEDTA	36.70	100.00
H3BO3	10.00	161.73
MnSO4.H2O	18.95	111.94
Na2MoO4.2H2O	0.25	1.03
ZnSO4.7H2O	10.00	34.78

Macro Elements	mg/l	mM
Ca(NO3)2.4H2O	Ommitted (500 mg/l)
KH2PO4	125.00	0.92
KNO3	125.00	1.24
MgSO4	61.00	0.51
Ca(NO3)2.4H2O	Ommitted (500 mg/l)
KH2PO4	125.00	0.92
KNO3	125.00	1.24
MgSO4	61.00	0.51

Total concentration Micro and Macro elements: 386.95 mg/l

Total concentration Micro and Macro elements: 386.95 mg/l

Lichter, R., Z. Planzephysiol., 103, 229-237, 1981, Lichter, R., Z. Planzephysiol., 105, 427-434, 1982

Lichter, R., Z. Planzephysiol., 103, 229-237, 1981, Lichter, R., Z. Planzephysiol., 105, 427-434, 1982