MycoReal® Kit Nosema apis & ceranae

MycoReal® Kit Nosema apis & ceranae

Product description

Kit contents:

• Detection assay for Nosema apis
• Detection assay for Nosema ceranae
• Detection assay for internal positive control (control of amplification)
• DNA reaction mix (contains uracil-N glycosylase, UNG)
• Positive controls for Nosema apis and Nosema ceranae
• Water

Background: Nosema apis and Nosema ceranae are microsporidian parasites recently reclassified as a fungus. Both species cause nosema disease in honey bees (Apis mellifera). The dormant stages are long-lived spores. The most notable symptom is dysentery. Nosema ceranae can be detected in all four seasons, while Nosema apis occurs mostly in the milder seasons of autumn and spring. There is a higher mortality of bees when they are infected by Nosema ceranae than when infected with Nosema apis. Double infection is also possible.

Description: BactoReal^® Kit Nosema apis & ceranae is based on the detection and differentiation of the 18S rRNA gene of Nosema apis (detection in VIC/HEX channel) as well as of the 18S rRNA gene of Nosema ceranae (detection in FAM and VIC/HEX channel) using multiplex real-time PCR. Thus, samples showing a positive signal in FAM as well as in VIC/HEX channel are positive for N. ceranae. Samples showing a positive signal in VIC/HEX channel are positive for N. apis.

PCR-platforms: BactoReal^® Kit Nosema apis & ceranae is developed and validated for the ABI PRISM^® 7500 instrument (Life Technologies), LightCycler^® 480 (Roche) and Mx3005P^® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Sensitivity and specificity: See product description