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5-Bromo-4-chloro-3-indolyl-β-D-galactoside (X-Gal) research grade
Product code: SERV.1524303 SERV.1524303
X-gal is a well known histochemical substrate used to detect the β-galactosidase enzyme (1, 2, 3, 4, 9, 10). Identification of lacf-colonies (8). X-
Packaging Unit
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| ACTIVATE VARIANT | PACKAGING UNIT | PRICE | QUANTITY |
|---|---|---|---|
| SERV.1524303 |
1 G |
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| SERV.1524306 |
250 MG |
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| SERV.1524307 |
5 G |
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5-Bromo-4-chloro-3-indolyl-β-D-galactoside (X-Gal) research grade
Product description
X-gal is a well known histochemical substrate used to detect the ž-galactosidase enzyme (1, 2, 3, 4, 9, 10). Identification of lacf-colonies (8). X-gal is used to distinguish recombinant plasmids from parental vectors in cloning experiments using vectors containing the lacZ or lacZ [DMvP1](-)peptide gene (2, 3, 5). Upon hydrolysis, X-gal yields a localized, insoluble blue precipitate making it exceptionally useful in blotting, immunocytochemical, and ELISA assays. X-gal has been used for the detection of coliforms (E. coli) in municipal water supplies (6) and food products (7). X-gal is often used in conjunction with IPTG (Isopropyl-ž-D-thiogalactopyranoside, cat. no. 26600) which binds and inhibits the lac repressor thus inducing ž-galactosidase activity.Stock solution: 20 mg/ml in DMF. Store in aliquots at -20 ’C in the dark. For detection of transformants, use in final concentration of 40 ’…g/ml.
5-Bromo-4-chloro-3-indolyl-β-D-galactoside (X-Gal) research grade
Product specifications
CAS No.
7240-90-6
Packaging Unit
1 G
Brand
SERVA
Shipment conditions
15 °C - 30 °C
Stock temperature condition
+2 °C TO +8 °C
Warning
5-Bromo-4-chloro-3-indolyl-β-D-galactoside (X-Gal) research grade
Product documents