Taq DNA Polymerase

Taq DNA Polymerase

Product description

highQu Taq DNA Polymerase is the classical enzyme for routine PCR applications providing high amplification yields of 3-5 kb targets under various conditions. Taq DNA Polymerase is purified from a recombinant E. coli strain carrying the Taq DNA polymerase gene.

Taq DNA polymerase is thermostable 5´ → 3´ DNA polymerase. It lacks 3´ → 5´ exonuclease (proofreading) activity and has low 5´ → 3´ exonuclease activity. Polymerase exhibits deoxynucleotidyl transferase activity resulting in A-overhang at the 3'-ends of PCR products and allowing for TA cloning. The PCR accuracy of Taq DNA Polymerase is 4.5 x 10⁴ (a number of incorporated nucleotides before the first error occurs).

The supplied reaction buffer for most flexibility in optimization, contains no dNTPs and no magnesium. The 50 mM MgCl₂ solution is provided separately what allows for magnesium optimization starting from 1 mM up to 4 mM final concentration upon the need. The dNTPs in sets or mixes can be purchased from highQu separately.

Applications

• Routine PCR up to 5 kb under common and fast conditions
• RT-PCR, colony PCR,TA cloning
• Library construction, genotyping, screening

Benefits

• High yields in routine PCR and under fast cycling conditions
• Robust performance under variety of conditions
• Excellent performance on complex (GC rich) templates