BactoReal® Kit American Foulbrood

BactoReal® Kit American Foulbrood

Product description

Kit contents:

• Detection assay for Paenibacillus larvae
• Detection assay for internal positive control (control of amplification)
• DNA reaction mix (contains uracil-N glycosylase, UNG)
• Positive control for Paenibacillus larvae
• Water

Background: American foulbrood (AFB) is a bacterial brood disease of honey bees caused by the Gram-positive and spore-forming bacterium Paenibacillus larvae. The disease is transferred and initiated only by the spore stage of the bacterium. Young bee larvae are infected by spores, then the spores germinate in the gut of the larva and the vegetative form of the bacteria begins to grow, taking its nourishment from the larva. Infected larvae normally die after their cell is sealed. The vegetative form of the bacterium produces many millions of spores which may remain dormant for many years.

Description: BactoReal^® Kit American Foulbrood is based on the amplification and detection of the 16S rRNA gene of Paenibacillus larvae using real-time PCR.

PCR-platforms: BactoReal^® Kit American Foulbrood is developed and validated for the ABI PRISM^® 7500 instrument (Life Technologies), LightCycler^® 480 (Roche) and Mx3005P^® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Sensitivity and specificity: See product description